|
KMID : 0368420090520010065
|
|
Journal of Plant Biology
2009 Volume.52 No. 1 p.65 ~ p.72
|
|
|
Identification and Functional Analysis of Phosphoproteins Regulated by Auxin in Arabidopsis Roots
|
|
Yi Tingfang
Dong Gaofeng
Wang Zenglan
Li Jiaxu
|
|
|
Abstract
|
|
|
|
The phytohormone auxin modulates diverse aspects of plant growth and development. Protein phosphorylation is believed to play a key role in regulating auxin-mediated responses. To determine the phosphoproteins affected by auxin in Arabidopsis, we used phospho-specific antibodies to analyze their profiles on two-dimensional gels, then identified them by mass spectrometry. We found two phosphoproteins, enolase and the beta subunit of succinyl-CoA synthetase (SCS-beta), and noted that their phosphorylation was increased by auxin. To investigate their importance in auxin-mediated processes, we characterized Arabidopsis knockout mutants of the two genes. A homozygous null mutation in the gene for SCS-beta conferred embryo lethality. The enolase knockout mutants showed defects in root development similar to those of auxin-related mutants such as alf3 and xbat32. Therefore, we suggest that enolase is involved in auxin-regulated processes.
|
|
|
KEYWORD
|
|
|
Arabidopsis, Auxin, Enolase, Phosphorylation, Succinyl-CoA synthetase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|